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tissue fragments (MedChemExpress)

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MedChemExpress tissue fragments
Tissue Fragments, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+fragments/Collagenase+IV%2C+Clostridium+histolytica/10__1016_slash_j__jot__2025__101032-118-0-7
Average 95 stars, based on 21 article reviews

tissue fragments - by Bioz Stars, 2026-09

95/100 stars

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Article Title: LARP1 acts as a key mediator in preventing angiotensin II-induced cardiac dysfunction and fibrosis
Article Snippet: The tissue fragments were digested with 0.1% trypsin (HY-129047; MedChemExpress) for 15 min. To terminate the enzymatic reaction, 10 mL of PBS was added.

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Article Title: Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury
Article Snippet: .. Tissue fragments were digested with collagenase (HY-E70005D, MCE, USA) and DNase (HY-108882, MCE, USA) at 37 °C for 1 h, followed by filtration through a 70um cell strainer (352,350, BD Biosciences). .. For pericyte isolation, cells were incubated with PE anti-mouse CD140b (1:100, 12-1402-81, Invitrogen, USA) and APC anti-mouse CD13 (1:100, 164,005, BioLegend, USA) on ice for 30 min. To further discriminate pericyte subpopulations, cells were fixed and permeabilized, then stained with CoraLite® Plus 488-conjugated smooth muscle actin antibody (1:50, CL488-80008, Proteintech, China), allowing separation of Pdgfrβ + Acta2 + and Pdgfrβ + Acta2 - populations.

Article Title: Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury
Article Snippet: .. Tissue fragments were digested with collagenase (HY-E70005D, MCE, USA) and DNase (HY-108882, MCE, USA) at 37 ◦ C for 1 h, followed by filtration through a 70um cell strainer (352,350, BD Biosciences). .. For pericyte isolation, cells were incubated with PE anti-mouse CD140b (1:100, 12-1402-81, Invitrogen, USA) and APC anti-mouse CD13 (1:100, 164,005, BioLegend, USA) on ice for 30 min. To further discriminate pericyte subpopulations, cells were fixed and permeabilized, then stained with CoraLite® Plus 488-conjugated smooth muscle actin antibody (1:50, CL488-80008, Proteintech, China), allowing separation of Pdgfrβ + Acta2 + and Pdgfrβ + Acta2 - populations.

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